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| Registros recuperados: 87 | |
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| AMORIM,MARÚCIA I. M.; MERGLER,DONNA; BAHIA,MARCELO O.; DUBEAU,HÉLÈNE; MIRANDA,DANIELA; LEBEL,JEAN; BURBANO,ROMMEL R.; LUCOTTE,MARC. |
| The mercury rejected in the water system, from mining operations and lixiviation of soils after deforestation, is considered to be the main contributors to the contamination of the ecosystem in the Amazon Basin. The objectives of the present study were to examine cytogenetic functions in peripheral lymphocytes within a population living on the banks of the Tapajós River with respect to methylmercury (MeHg) contamination, using hair mercury as a biological indicator of exposure. Our investigation shows a clear relation between methylmercury contamination and cytogenetic damage in lymphocytes at levels well below 50 micrograms/gram, the level at which initial clinical signs and symptoms of mercury poisoning occur. The first apparent biological effect with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian Amazon; Mercury; Mitotic Index; Cytogenetics. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000400004 |
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| Anjos,Allison; Loreto,Vilma; Souza,Maria José de. |
| In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3+ blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3+ blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2,S9 and S10... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; FISH; Fluorochromes; Grasshoppers; RDNA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300006 |
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| Azevedo,MFC; Foresti,F.; Ramos,PRR; Jim,J. |
| Specimens of Bufo ictericus, Bufo paracnemis and a third type, considered an intermediate subgroup between these species, were cytogenetically studied by conventional Giemsa staining, C-banding and staining of the nucleolus organizer region (NOR). The nuclear DNA content and seroproteins were also analyzed to characterize these species, and verify the possibility of hybridization between them. Karyotypes and cytogenetic markers were essentially equal on the basis of the methods used. The DNA nuclear content found was 6.25 ± 0.30 pg/DNA in Bufo ictericus; 7.57 ± 0.40 pg/DNA in Bufo paracnemis and 7.04 ± 0.29 pg/DNA in the intermediate subgroup. Eletrophoresis of total blood serum in Bufo ictericus, Bufo paracnemis and the intermediate specimens revealed a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bufo; Hybridization; Cytogenetics; Nuclear DNA; Seroproteins. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300012 |
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| Barranco, P.; Cabrero, J.; Camacho, J.P.M.; Pascual, F.. |
| Morphological and cytogenetic analyses of a spontaneous gynandromorph of Pycnogaster inermis, found in a natural population in the Spanish Sierra Nevada, have shown that the left side was male and the right side female, with almost perfect bilateral symmetry. Ventral valves of the ovipositor were very short, appearing as two spurs; dorsal valves were longer but still shorter than normal. The specimen had an apparently normal testis on the left side and a well-developed ovary on the right side. Cytogenetic analysis revealed the presence of 2n = 28 + X0 chromosomes in the testis and 2n = 28 + XX in the ovary, the same as standard males and females, respectively, of this species. This suggests that the gynandromorphism occurred at a very early developmental... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Gynandromorphism; Cytogenetics; Pycnogaster inermis; Tettigoniidae; Orthoptera. |
| Ano: 1995 |
URL: http://www.repository.naturalis.nl/record/504051 |
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| Barranger, Audrey; Akcha, Farida; Rouxel, Julien; Brizard, Raphael; Maurouard, Elise; Pallud, Marie; Menard, Dominique; Tapie, N.; Budzinski, Helene; Burgeot, Thierry; Benabdelmouna, Abdellah. |
| Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland. The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg... |
| Tipo: Text |
Palavras-chave: Herbicide; Diuron; Oyster; Genotoxicity; Comet assay; Cytogenetics. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00162/27311/25541.pdf |
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| Barth,Adriane; Fernandes,Anderson; Pompolo,Silvia das Graças; Costa,Marco Antônio. |
| Tetragonisca angustula and Tetragonisca fiebrigi have recently been listed as valid species. This study aimed to cytogenetically investigate both species, emphasizing the new registry of B chromosomes in the tribe Meliponini. We analyzed colonies of T. angustula and T. fiebrigi collected at Tangará da Serra, Mato Grosso, Brazil, through conventional Giemsa staining, C-banding, and base-specific fluorochrome staining (CMA3/DAPI). T. angustula showed 2n = 34 chromosomes in females and n = 17 in males, with karyotype formula 2K = 34A M. T. fiebrigi showed numeric variation, with chromosome number varying from 2n = 34 to 2n = 36 in females and from n = 17 to n=18in males, with karyotype formula 2K = 32A M+2A Mc and 2K = 32A M+2A Mc + 1 or 2 B-chromosomes. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stingless bees; Cytogenetics; Interspecific differentiation; B chromosomes. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100014 |
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| Bellafronte,Elisangela; Mariguela,Tatiane Casagrande; Pereira,Luiz Henrique Garcia; Oliveira,Claudio; Moreira-Filho,Orlando. |
| In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apareiodon; COI gene; Cytogenetics; Fish identification; Morphology. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300497 |
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| Bellafronte,Elisangela; Schemberger,Michelle Orane; Artoni,Roberto Ferreira; Moreira Filho,Orlando; Vicari,Marcelo Ricardo. |
| Parodontidae fish show few morphological characteristics for the identification of their representatives and chromosomal analyses have provided reliable features for determining the interrelationships in this family. In this study, the chromosomes of Apareiodon hasemani from the São Francisco River basin, Brazil, were analyzed and showed a karyotype with 2n = 54 meta/submetacentric chromosomes, and a ZZ/ZW sex chromosome system. The study revealed active NORs located on pair 11 and additional 18S rDNA sites on pairs 7 and 22. The 5S rDNA locus was found in pair 14. It showed a pericentric inversion regarding the ancestral condition. The satellite DNA pPh2004 was absent in the chromosomes of A. hasemani, a shared condition with most members of Apareiodon.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; FISH; 18S rDNA; 5S rDNA; Neotropical fishes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500010 |
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| Bitencourt,Jamille de Araújo; Affonso,Paulo Roberto Antunes de Mello; Giuliano-Caetano,Lucia; Dias,Ana Lucia. |
| Few chromosomal reports are available for the endemic fish fauna from coastal basins in northeastern Brazil, and regional biodiversity remains partially or completely unknown. This is particularly true for Loricariidae, the most diverse family of armored catfishes. In the present work, allopatric populations of Hypostomus cf. wuchereri (Siluriformes: Loricariidae) from two basins in Bahia (northeastern Brazil) were cytogenetically analyzed. Both populations shared 2n = 76 chromosomes, a karyotype formula of 10m+18sm+48st/a (FN = 104) and single terminal GC-rich NORs on the second metacentric pair. Nevertheless, microstructural differences were detected by C-banding, fluorochrome staining and chromosomal digestion with restriction enzymes (Alu I, Bam HI,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodiversity; Cytogenetics; Heterochromatin; Hypostominii. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000200008 |
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| Carrara,Rita C. V.; Yamasaki,Rui; Mazucatto,Luís F.; Veludo,Maria A. Llorach; Sartorato,Edi L.; Pina-Neto,João M.. |
| Clinical and cytogenetic studies were performed in 65 infertile individuals, and 56 of them were also screened for microdeletions in Yq11 (AZF region). Relevant environmental etiological factors were identified in 10 cases (15.4%). Sertoli-cell-only syndrome was diagnosed in six patients (9,2%). Karyotype abnormalities were detected in six individuals, and five other patients presented desynapsis of bivalents in meiosis. Three out of the 56 patients studied were carriers of microdeletions in the AZF region, one of them also presenting a chromosomal mosaicism for an extra i(22p). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Male infertility; Cytogenetics; Microdeletions; AZF; Meiotic anomalies. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400002 |
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| Carvalho de Souza,Patrícia; Salim Khayat,André; Chamon Seligmann,Igor; Rodríguez Burbano,Rommel Mario. |
| The collared peccary (Tayassu tajacu) is widely distributed over the American continent, being found from the south of the USA to the north of Argentina. In Brazil, it is spread all over the country, being one of the potential species to be raised in captivity. Therefore, the cytogenetic techniques could be a potential tool for reproductive monitoring of animals raised in captivity, mainly when destined for commercial purposes. This study had the objective of determining the chromosome number of two populations raised in captivity and characterizing them by GTG banding. For this purpose, an analysis was made of mitotic metaphases obtained from lymphocyte cultures made from blood samples of 11 animals, six of which from the Northeast and five from the North... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cytogenetics; GTG Banding; Tayassuidae; Suidae; Pigs. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200007 |
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| Carvalho,Rafael Augusto de; Sanchez,Sebástian; Swarça,Ana Claudia; Fenocchio,Alberto Sergio; Martins-Santos,Isabel C.; Dias,Ana Lúcia. |
| This study presents chromosomal data of Megalonema platanum from rio Tibagi, Paraná, Brazil and from rio Paraná, Argentina. The diploid number was equal 54 with karyotype composition of 24m+16sm+2st+12a in both populations. The AgNOR sites were detected in the terminal position of a submetacentric pair of the two analyzed populations, coinciding with secondary constrictions on the short arm of pair 15. CMA3 and FISH with 18S rDNA probe displayed fluorescent signals that correspond to the AgNOR sites and secondary constriction. The presence of a small acrocentric supernumerary chromosome can be observed in M. platanum from rio Tibagi, with centromeric heterochromatin. Others heterochromatic blocks were evidenced in the terminal position of some chromosome... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; C-banding; CMA3; FISH; NORs; Pimelodidae. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100017 |
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| Castanhole,Márcia Maria Urbanin; Pereira,Luis Lênin Vicente; Souza,Hederson Vinicius de; Valério,José Raul; Barbosa,Leonardo Rodrigues; Itoyama,Mary Massumi. |
| Spittlebugs annually infest pastures and cause severe damage, representing a serious problem for the tropical American beef cattle industry. Spittlebugs are an important biotic constraint to forage production and there is a lack of cytogenetic data for this group of insects. For these reasons, we conducted this work, in which the spermatogenesis and nucleolar behavior of Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana were studied. The males possessed testes in the shape of a "bunch of grapes"; a variable number of testicular lobes per individual and polyploid nuclei composed of several heteropycnotic bodies. A heteropycnotic area was located in the periphery of the nucleus (prophase I); the chiasmata were terminal or interstitial;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosomal characterization; Karyotype; Cytogenetics; Spermatogenesis; Holocentric chromosomes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200008 |
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| Registros recuperados: 87 | |
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